DEPARTMENT OF MICROBIOLOGY
GMERS MEDICAL COLLEGE, GODHRA, PANCHMAHAL
Overview
The broad goal of the teaching of undergraduate students in Microbiology is to provide an understanding of the natural history of infectious disease in order to deal with the aetiology, pathogenesis, laboratory diagnosis, treatment and control of infections in the community
KNOWLEDGE
At the end of the course, the student should be able to:
1. State the infective micro-organisms of the human body and describe the host parasite relationship.
2. List pathogenic micro-organisms (bacteria, viruses, parasites, fungi) and describe the pathogenesis of the diseases produced by them.
3. State or indicate the modes of transmission of pathogenic and opportunistic organisms and their sources, including insect vectors responsible for transmission of infection.
4. Describe the mechanisms of immunity to infections.
5. Acquire knowledge on suitable antimicrobial agents for treatment of infections and scope of immunotherapy and different vaccines available for prevention of communicable diseases.
6. Apply methods of disinfection and sterilization to control and prevent hospital and community acquired infections.
7. Recommend laboratory investigations regarding bacteriological examination of food, water, milk and air.
SKILLS
At the end of the course, the student should be able to:
1. Plan and interpret laboratory investigations for the diagnosis of infectious diseases and to correlate the clinical manifestations with the etiological agent.
2. Identify the common infectious agents with the help of laboratory procedures and use antimicrobial sensitivity tests to select suitable antimicrobial agents.
3. Perform commonly employed bed-side tests for detection of infectious agents such as blood smear for malaria, filaria, gram staining and AFB staining and stool sample for ova, cyst.
4. Use the correct method of collection, storage and transport of clinical material for microbiological investigations.
INTEGRATION
The student should understand infectious diseases of national importance in relation to the clinical, therapeutic and preventive aspects.
Facilities
1) UNDER GRADUATE LABORATORY
LIST OF INSTRUMENTS IN Undergraduate LABORATORY / RESEARCH LABORATORY
|
Sr. No. |
Name of equipment |
Quantity |
|
1 |
Hot air oven |
02 |
|
2 |
B. O. D Incubator |
02 |
|
3 |
Microscope |
70 |
|
4 |
Deep fridge |
01 |
|
5 |
Refrigerator |
01 |
|
6 |
Centrifuge |
02 |
|
7 |
Elisa Reader |
01 |
2) CLINICAL LABORATORY
A) LIST OF EQUIPMENTS IN CLINICAL LABORATORY
|
Sr. No. |
Name of equipment |
Name of Company |
Quantity |
|
1 |
Centrifuge |
Remi Neaution |
03 |
|
2 |
Refrigerated Centrifuge |
Remi Newton |
03 |
|
3 |
Bio Safety Cabinet |
One Gene Mediscan |
04 |
|
4 |
Vortex Mixture |
Remi |
02 |
|
5 |
Auto Clave |
Swastik Meta |
03 |
|
6 |
Fluorscent Qualitative Detection System |
Himedia |
01 |
|
7 |
RTPCR Machine |
Remi Bio Rad |
02 |
|
8 |
Water Bath |
Labtech |
01 |
|
9 |
Deep Fridge |
Elanpro RA Celfrost |
04 |
|
10 |
Elisa Reader |
Tullip Lisa Quant Lisa Scan |
03 |
|
11 |
Elisa Washer |
Tullip Lisawasher |
02 |
|
12 |
Bio Tech Machine |
Bactec |
01 |
|
13 |
Microscope |
Olympus Den Megna |
04 |
|
14 |
Refrigerator |
Lg Haier |
02 |
|
15 |
Thermometer |
|
04 |
B) Scope available at facility:
|
Sr No |
Name of the test |
Test Principal |
|
Bacteriology |
||
|
1 |
Aerobic culture and Sensitivity testing ( Biological samples) |
Conventional culture and AST |
|
2 |
Diphtheria Culture |
Conventional |
|
3 |
Anaerobic culture |
Conventional |
|
4 |
Automated Blood Culture |
BD Bactec system for Automated Bi culture |
|
5 |
Gram Staining |
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|
6 |
ZN staining |
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|
7 |
Albert Staining |
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|
8 |
Giemsa staining |
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|
9 |
ZN stain for Lepra bacilli |
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|
10 |
Environmetal surveillance |
Air, Water and Surface Samples culture |
|
Serology |
||
|
11 |
HbsAg detection |
ELISA |
|
12 |
HbsAg detection |
Immunochromatography |
|
13 |
ASO |
Latex agglutination |
|
14 |
RA factor |
Latex agglutination |
|
15 |
CRP |
Latex agglutination |
|
16 |
WIDAL slide and tube |
Latex agglutination |
|
17 |
RPR |
Passive agglutination |
|
18 |
TPHA for Syphilis |
Passive agglutination |
|
|
|
|
|
Virology |
||
|
19 |
NS1 ag detection for Dengue |
ELISA |
|
20 |
IgM anti Dengue Ab |
ELISA |
|
21 |
IgM anti CHIK Ab |
ELISA |
|
22 |
IgM anti HAV Ab |
ELISA |
|
23 |
IgM anti HEV Ab |
ELISA |
|
24 |
Anti HCV Ab |
ELISA |
|
25 |
Anti HCV Ab |
Immunochromatography |
|
ICTC & Immunology |
||
|
34 |
Anti HIV antibody |
ELISA |
|
35 |
Anti HIV antibody |
Rapid |
|
36 |
DBS |
- |
|
Mycology |
||
|
37 |
Microscopy for Fungal identification |
|
|
38 |
KOH |
|
|
39 |
India ink |
|
|
40 |
Fungal Culture |
|
|
|
||
|
41 |
Stool microscopy |
Wet mount |
|
42 |
Modified ZN stain for parasites |
|
|
Molecular |
||
|
43 |
Influenza |
RT PCR |
|
44 |
COVID 19 |
RT PCR |
|
Outbreak investigation |
||
|
AMR surveillance |
||
Department Of Microbiology - Diagnostic & Teaching facility
TESTS DONE IN VARIOUS LABS
Serology –
ELISA for Hepatitis B surface antigen, Measles IgM antibody, Brucella IgM antibody
Rapid tests – hepatitis B surface antigen, RA factor for Rheumatoid arthritis, CRP, ASO test for rheumatic fever , Widal test for Typhoid, RPR & TPHA test for syphilis
Virology –
ELISA for Dengue NS1 Ag, DengueIgM antibody, hepatitis C, Hepatitis A, Hepatitis E, Chikungunya IgM antibody,Toxoplasma IgM & IgG antibody, Herpes IgM & IgG antibody,
Rapid test- Hepatitis C antibody
Immunology – HIV antibody ELISA & rapid, DBS collection for HIV DNA PCR
Bacteriology –
Microscopy
wet mount,stain- Gram, ZN for Mycobacterium tuberculosis, Albert stain for Diphtheria ,Giemsa, etc
Culture & antibiotic susceptibility testing for diagnosis of infectious diseases like Cholera, Typhoid, meningitis, UTI, Pneumonia, Septicaemia, Diphtheria, Dysentery, Pharyngitis etc (from Pus, Blood, Urine, CSF, Sputum, Stool, fluids,Tissue etc)
Parasitology –
Diagnosis of parasitic diseases like Amoebiasis, intestinal worms, Filariasis etc by Stool routine Micro
Modified ZN stain for stool parasites
Mycology –
Diagnosis of Fungal infections of skin, hair, nail ,ear, eyes etc by Microscopy(KOH) & culture
Molecular –
Diagnosis of various viral infections like COVID 19 by RT PCR
Infection control –
surveillance of Air, Water &surface in hospital for infection control & prevention AMR surveillance
National viral hepatitis control program(NVHCP) for Hepatitis B & Hepatitis C National program for surveillance of Viral Hepatitis (NPSVH)
National AIDS control program – Viral load testing for HIV 1by RT PCR
District Public health lab (DPHL) – Samples received from Godhra rural & Panchmahal, Mahisagar Outbreak investigation
i. Demonstration room: 02
ii. Practical class room/laboratories: 01
iii. Departmental library-cum-seminar room: 01
iv. Research laboratories: 01
v. Office accommodation: 01
Faculty details in department of MICROBIOLOGY
|
Sr No. |
Name of faculty |
Designation |
|
1 |
Dr. Rakesh Rajat M.B.B.S MD (Microbiology) Email: rakeshrjt@gmail.com |
Professor & Head |
|
2. |
Dr. Jignasha Tadvi M.B.B.S MD (Microbiology) Email: dr.jignasha.tadvi@gmail.com |
Associate Professor |
|
3. |
Dr. Neela Katara M.B.B.S MD (Microbiology) Email: kataraneela8@gmail.com |
Assistant Professor |
|
4. |
Dr. Mihir Oza M.B.B.S MD (Microbiology) Email: mihiroza1695@gmail.com |
Assistant Professor |
|
5 |
Dr Priyal Patel M.B.B.S MD (Microbiology) Email: priyalmpatel007@gmail.com |
SR |
|
6 |
Dr Prarthna Patel M.B.B.S Email: prarthanapatel01@gmail.com |
JR |
|
7 |
Dr Helly Patel M.B.B.S Email: hellypatel762@gmail.com |
JR |
(VI)LIST OF BOOKS IN DEPARTMENTAL LIBRARY:
|
Sr. No. |
Name of Books |
Author |
Quantity |
|
1 |
Apurba Sastry Essentials of Medical Microbiology
|
Apurba Sastry & Sandhya Bhat |
02 |
|
2 |
Ananthanarayan and Paniker's Textbook of Microbiology
|
R Ananthnarayan & C.K Jayram Paniker |
04 |
|
3 |
C.P. Baveja Textbook of Microbiology |
C.P. Baveja |
02 |
|
4 |
Laboratory record book in Microbiology |
|
01 |
Subject Curriculum: Department of Microbiology is teaching 2nd year MBBS students as per CBME curriculum.
|
CBME based UG Curriculum Topic name of Theory Class:
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SECTION 1: General Microbiology
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General Microbiology Microscopy General Bacteriology Bacterial Taxonomy Morphology and Physiology of Bacteria Laboratory Diagnosis of Bacterial Infections Specimen Collection Direct Detection (Staining Methods and Others) Culture, Identification and Antimicrobial Susceptibility Test Molecular Diagnosis and Typing Methods Bacterial Genetics- Antimicrobial Agents and Antimicrobial Resistance Pathogenesis of Bacterial Infections Overview of Bacterial Infections. General Virology and Overview of Viral Infections. General Parasitology and Overview of Parasitic Infections General Mycology and Overview of Fungal Infections Normal Human Microbiota Epidemiology of Infectious Diseases
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SECTION 2: Immunology
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Immunity (Innate and Acquired) Antigen Antibody Antigen-antibody Reaction Complement Components of Immune System: Organs, Cells and Products Immune Responses: Cell-mediated and Antibody-mediated Hypersensitivity Autoimmunity Immunodeficiency Disorders Transplant and Cancer Immunology Immunoprophylaxis
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SECTION 3: Hospital Infection Control
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Healthcare-associated Infections Major Healthcare-associated Infection Types Sterilization and Disinfection Biomedical Waste Management Needle Stick Injury Antimicrobial Stewardship Environmental Surveillance (Bacteriology of Water, Air and Surface)
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SECTION 4: Bloodstream and Cardiovascular System Infections
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Cardiovascular System Infections: Infective Endocarditis and Acute Rheumatic Fever Bloodstream Infections (Including Infections Causing Anemia) Bacterial Infections Enteric Fever (Salmonella Typhi and Salmonella Paratyphi Rickettsial Infections Miscellaneous Bacterial Bloodstream Infections: Brucellosis, Leptospirosis and Borreliosis Viral Infections HIV/AIDS Viral Hemorrhagic Fever (VHF) Arboviral VHF (Dengue, Chikungunya and Others), Filoviral VHF (Ebola and Marburg Virus), Hantaviral and Other-Agents of VHF Parasitic Infections Malaria and Babesiosis Visceral Leishmaniasis and Trypanosomiasis Lymphatic Filariasis Fungal Infections Systemic Candidiasis and Systemic Mycoses
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Section 5: Gastrointestinal (GI) Infections
Gastrointestinal Infective Syndromes Bacterial Infections Food Poisoning: S. aureus, Bacillus cereus, Clostridium botulinum and Others Gastrointestinal Infections due to Enterobacteriaceae: Diarrheagenic Escherichia coli, Shigellosis, Nontyphoidal Salmonellosis and Yersiniosis Cholera, Halophilic Vibrio and Aeromonas Infections Miscellaneous Bacterial Infections of Gastrointestinal System: Helicobacter, Campylobacter and Clostridioides difficile Infections Viral Infections Viral Gastroenteritis: Rotaviruses and Others Parasitic Infections Intestinal Protozoan Infections: Intestinal Amoebiasis, Giardiasis, Coccidian Parasitic Infections, Balantidiasis, Blastocystosis, and Others Intestinal Helminthic Infections Intestinal Cestode Infections: Diphyllobothrium, Taenia, Hymenolepis and Others Intestinal Trematode Infections: Fasciolopsis buski, Schistosoma mansoni, S. japonicum and Others Intestinal Nematode Infections: Trichuris, Enterobius, hookworm, Strongyloides, Ascaris and Others
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Section 6:Hepatobiliary System Infections Infective Syndromes of Hepatobiliary System and Abdomen Viral Infections Viruses Causing Hepatitis Hepatitis Viruses, Yellow Fever and Others Parasitic Infections Parasitic Infections of Hepatobiliary System Amoebic Liver Abscess, Hydatid Disease (Echinococcosis), Trematode Infections (Fasciola hepatica, Clonorchis and Opisthorchis) and Others |
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Section 7: Skin, Soft Tissue and Musculoskeletal System Infections
Infective Syndromes of Skin, Soft Tissue and Musculoskeletal Systems Bacterial Infections Staphylococcal Infections Beta-hemolytic Streptococcal Infections Gas Gangrene (Clostridium perfringens) and Infections due to Non-sporing Anaerobes Leprosy (Mycobacterium leprae) and Others Anthrax (Bacillus anthracis), Actinomycosis, Nocardiosis, Nonvenereal Treponematoses Miscellaneous Bacterial Infections of Skin and Soft Tissues: Viral Exanthems and Other Cutaneous Viral Infections Viral Infections Herpesviruses (Herpes Simplex, Varicella-zoster and HHV-6 and 7 infection). Poxviruses (Smallpox, Molluscum contagiosum), Parvovirus, Measles, Rubella, Coxsackieviruses and Others Parasitic Infections Parasitic Infections of Skin, Soft Tissue and Musculoskeletal System Cutaneous Leishmaniasis, Cysticercosis, Tissue Nematodes (Filarial Tissue Nematodes, Dracunculus medinensis, Trichinella spiralis) and Larva Migrans Fungal Infections Fungal Infections of Skin, Soft Tissue and Musculoskeletal System Superficial Fungal Infections, Subcutaneous Fungal Infections, Candidiasis (cutaneous and mucosal) and Penicillium marneffei Infection |
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Section 8: Respiratory Tract Infections
Infective Syndromes of Respiratory Tract Bacterial Pharyngitis: Streptococcus pyogenes Pharyngitis, Diphtheria and Bacterial Infection Bacterial Lobar Pneumonia: Pneumococcal Pneumonia, Haemophilus influenzae Pneumonia and Others Bacterial Atypical (Interstitial) Pneumonia: Mycoplasma, Chlamydia, Legionella and Others Tuberculosis and Nontuberculous Mycobacteria Infections Pertussis (Bordetella pertussis) Infections due to Non-fermenting Gram-negative Bacilli: Pseudomonas, Acinetobacter, Burkholderia and Others Viral Infections Myxovirus Infections of Respiratory Tract: Influenza, Parainfluenza, Mumps, Respiratory Syncytial Virus and Others Coronavirus Infections Including COVID-19 Miscellaneous Viral Infections of Respiratory Tract: Rhinovirus, Adenovirus and Infectious Mononucleosis (Epstein-Barr Virus) Parasitic and Fungal Agents Parasitic and Fungal Infections of Respiratory Tract Parasitic Infections: Paragonimiasis and Others Fungal Infections: Zygomycosis, Aspergillosis, Pneumocystosis and Others |
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Section 9: Central Nervous System Infections
Infective Syndromes of Central Nervous System Bacterial Infections Bacterial Meningitis Acute Bacterial (Pyogenic) Meningitis: Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae, Haemophilus influenzae and Listeria Chronic Bacterial Meningitis: Tubercular Meningitis, Spirochetal Meningitis, Lyme Disease and Others Tetanus Viral Infections Viral Meningitis and Myelitis: Poliomyelitis, Coxsackievirus Infections, and Others Viral Encephalitis and Encephalopathy Rabies, HSV Encephalitis, Arboviral Encephalitis (Japanese Encephalitis and West Nile). Nipah and Hendra, Slow Virus and Prion Disease, and Others Parasitic and Fungal Infections Parasitic and Fungal Infections of Central Nervous System Parasitic Infections: Neurocysticercosis, Free-living Amoeba Infections, Toxoplasmosis and Others Fungal Infections: Cryptococcal Meningitis and Others |
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Section 10: Urogenital Tract Infections
Infective Syndromes of Urinary Tract Bacterial Infections: Enterobacteriaceae, Enterococcus and Others Viral (BK Virus), Parasitic (Schistosoma haematobium) and Fungal Infections. Infective Syndromes of Genital Tract and Sexually transmitted Infections Ulcerative Genital Disease: Syphilis, Lymphogranuloma Venerum, Granuloma Inguinale, Soft Chancre and Genital Herpes Gonorrhea and Non-gonococcal Urethritis (Chlamydia trachomatis and Others) Vulvovaginitis (Trichomoniasis, Bacterial Vaginosis, Vaginal Candidiasis) Other Genital Tract Infections of Females and Males |
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Section 11: Miscellaneous Infective Syndromes
Ocular and Ear Infections Congenital Infections Cytomegalovirus Infections, Congenital Varicella, Neonatal Herpes, Congenital Rubella, Congenital Toxoplasmosis, Congenital Syphilis, Zika Virus Infections and Others Organisms with Oncogenic Potential Human Papilloma Virus, Kaposi Sarcoma, HTLV and HIV, Epstein-Barr Virus, Hepatitis B and C, and Others Zoonotic Infections: Plague, Tularaemia ahd Bite Wound Infections |
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AETCOM in Microbilogy |
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SR. NO. |
Topic name of Practical Class: |
|
Bio-safety in health care practices to prevent 1 health care associated infections Sterilization & disinfection Microscope Staining methods and study of bacterial morphology Gram stain ZN stain Culture media Isolation of bacteria in laboratory Identification of bacteria Bacteria and infectious diseases Antimicrobial sensitivity Diagnosis of fungal infection Laboratory diagnosis of parasitic diseases 97 (Intestinal parasites) Laboratory methods for diagnosis of viral infections Molecular methods Immunological and serological tests Automation in Microbiology Approach for laboratory diagnosis of infectious diseases Blood culture methods and laboratory diagnosis of blood stream infections including infective endocarditis Laboratory diagnosis of rheumatic heart disease Laboratory diagnosis of enteric fever Laboratory diagnosis of malaria, filaria & other extra-intestinal parasites Laboratory diagnosis of HIV infection Laboratory diagnosis of Gastroenteritis Laboratory diagnosis of viral hepatitis Laboratory diagnosis of Skin & soft tissue infections Laboratory diagnosis of bone and joint Laboratory diagnosis of Meningitis Laboratory diagnosis of upper respiratory tract infections Laboratory diagnosis of lower respiratory tract infections Laboratory diagnosis of Tuberculosis Laboratory diagnosis of Urinary tract infections Laboratory diagnosis of STIs Laboratory diagnosis of Pyrexia (fever) of unknown origin Hospital infection control program & practices Confidentiality in lab reports |
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31 |
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32 |
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33 |
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34 |
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35 |
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36 |
Theory Exam Pattern
|
NO. |
Theory Paper (1 & 2) |
Total 100 Marks |
|
1 |
MCQ |
20 Marks |
|
2 |
Long Essay Question |
24 Marks |
|
3 |
Short Notes (applied) |
28 Marks |
|
4 |
Short Notes / AETCOM |
28 Marks |
|
Practical Exam Pattern
|
||
|
NO. |
Practical |
Total 100 Marks |
|
Question – 1 |
SPOTTING |
10 |
|
Question – 2 |
Case Base Study |
20 |
|
Question – 3 |
Viva -TABLE 1 |
25 |
|
Viva - TABLE 2 |
25 |
|
|
Question – 4 |
GRAM OR ZN EXERSISE |
20 |
PUBLICATION DETAILS
|
Sr. No. |
Faculty name |
Publication number |
|
1. |
Dr. Rakesh Rajat |
08 |
|
2. |
Dr. Jignasha Tadvi |
06 |
|
3. |
Dr. Neela Katara |
02 |
|
4. |
Dr. Mihir Oza |
02 |
|
5. |
Dr. Priyal Patel |
01 |
EVENTS
Department of Microbiology Conducted Activity on World Antimicrobial Awareness Week on 18th to 24th November 2024 at GMERS Medical College and General Hospital, Panchmahal, Godhra.
